rabbit polyclonal anti stim1  (Bioss)

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: STIM1 immunohistochemical expression in the studied groups. ( a ) A case of normal pancreatic tissue showed weak cytoplasmic STIM1 epithelial expression in the pancreatic duct and focal cytoplasmic STIM1 stromal expression (IHC x200), ( b ) A case of adjacent non-tumor pancreatic tissue showed moderate cytoplasmic STIM1 epithelial expression and focal STIM1 cytoplasmic stromal expression (IHC x100), ( c ) A case of control non-tumor intestinal tissue showed moderate cytoplasmic STIM1 epithelial expression and focal cytoplasmic STIM1 stromal expression (IHC x100), ( d ) A case of primary PDAC showed strong cytoplasmic STIM1 epithelial and stromal expression (IHC x100), ( e ) A case of metastatic PDAC to the liver (on the left side) showed strong cytoplasmic STIM1 epithelial and stromal expression (IHC x100), ( f ) A case of AAC showed strong cytoplasmic STIM1 epithelial and stromal expression (IHC x100)

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques: Immunohistochemical staining, Expressing, Control

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Comparison between STIM1 and SOX2 expression in PDAC and the control groups. ( a ) Comparison between STIM1 epithelial expression in PDAC and the control groups, ( b ) Comparison between STIM1 stromal expression in PDAC and the control groups, ( c ) Comparison between SOX2 epithelial expression in PDAC and the control groups, ( d ) Comparison between SOX2 stromal expression in PDAC and the control groups. ( e ) Comparison between STIM1 epithelial expression in AAC and the control groups. ( f ) Comparison between STIM1 stromal expression in AAC and the control groups. ( g ) Comparison between SOX2 stromal expression in PDAC and the control groups. ( h ) Comparison between SOX2 stromal expression in AAC and the control groups

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques: Comparison, Expressing, Control

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Relationship between STIM1 expression and clinicopathological data in the primary PDAC group (No = 48) and AAC (No = 21)

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques: Expressing

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Kaplan-Meier survival curve demonstrating the impact of clinicopathological and STIM1/SOX2 expressions on the overall survival of AAC cases

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques:

Journal: World Journal of Surgical Oncology

Article Title: STIM1/SOX2 proteins are co-expressed in the tumor and microenvironmental stromal cells of pancreatic ductal adenocarcinoma and ampullary carcinoma

doi: 10.1186/s12957-024-03356-y

Figure Lengend Snippet: Univariate and multivariate COX regression analysis for the parameters affecting morality in the primary AAC group

Article Snippet: Rabbit polyclonal anti-STIM1 diluted as 1:200 (Cat. No. bs-8526R) obtained from BIOSS, Woburn, Massachusett, USA, rabbit polyclonal anti-SOX2 diluted as 1:250 (Cat. No. GB11249) obtained from Service bio, Wuhan, China, and a rabbit monoclonal anti-BCL2 antibody ready to use, (Cat. No. ab32124) obtained from Abcam, Cambridge, UK were used.

Techniques:

Journal: The Journal of Biological Chemistry

Article Title: Enhanced Orai1-mediated store-operated Ca 2+ channel/calpain signaling contributes to high glucose-induced podocyte injury

doi: 10.1016/j.jbc.2022.101990

Figure Lengend Snippet:

Article Snippet: Rabbit polyclonal anti-STIM1 , Proteintech , Cat#: 11565-1-AP.

Techniques: Western Blot, Imaging, Staining, Plasmid Preparation, Activity Assay, Cell Culture

Journal: eLife

Article Title: STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells

doi: 10.7554/eLife.70278

Figure Lengend Snippet: ( A ) Representative Wes protein capillary electrophoresis experiments, presented as Western blots, showing STIM1 protein expression levels in smooth muscle tissues and brains of control and Stim1- smKO mice. Summary data showing densitometric analyses of STIM1 protein expression in cerebral artery smooth muscle (CA SM), mesenteric artery smooth muscle (MA SM), aortic smooth muscle, colonic smooth muscle, bladder smooth muscle, and brain, normalized to total protein (n = 3–6 mice/group; *p<0.05, unpaired t -test). ns, not significant. ( B ) Representative epifluorescence superresolution localization maps of isolated cerebral artery SMCs from control and Stim1- smKO mice immunolabeled for STIM1. Insets: enlarged areas highlighted by the white squares in the main panels. Scale bars: 3 µm (main panels) and 250 nm (inset panels). ( C ) Distribution plot of the surface areas of individual STIM1 clusters in cerebral artery SMCs isolated from control mice (n = 42,726 clusters from 18 cells from three mice). ( D ) STIM1 cluster density in cerebral artery SMCs isolated from control and Stim1- smKO mice (n = 18 cells from three mice/group; *p<0.05, unpaired t -test). Figure 1—source data 1. Individual data points and analysis summaries for datasets shown in .

Article Snippet: Antibody , Anti-Stim1 (rabbit polyclonal) , Sigma-Aldrich , Cat# S6072; RRID: AB_1079008 , (1:1000).

Techniques: Electrophoresis, Western Blot, Expressing, Isolation, Immunolabeling

Journal: eLife

Article Title: STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells

doi: 10.7554/eLife.70278

Figure Lengend Snippet: ( A ) Representative traces of spontaneous transient outward currents (STOCs) in cerebral artery smooth muscle cells (SMCs) from control and Stim1- smKO mice, recorded by perforated patch-clamp electrophysiology over a range of membrane potentials (−60 to 0 mV). ( B, C ) Summary data showing STOC frequency ( B ) and amplitude ( C ) (control, n = 13 cells from four animals; Stim1- smKO , n = 17 cells from five mice; *p<0.05, two-way ANOVA). ( D ) Representative traces of paxilline (1 μM)-sensitive BK currents in cerebral artery SMCs from control and Stim1- smKO mice, recorded by patch-clamping in conventional whole-cell mode during a series of command voltage steps (−100 to +100 mV). ( E ) Summary data for whole-cell BK currents (control, n = 6 cells from three mice; Stim1- smKO, n = 7 cells from three mice; two-way ANOVA). ( F ) Representative traces of TRPM4 currents in cerebral artery SMCs from control and Stim1- smKO mice voltage-clamped at –70 mV, recorded using perforated patch-clamp electrophysiology. TRPM4 currents were evoked as transient inward cation currents (TICCs) by application of negative pressure (–20 mmHg) through the patch pipette and were blocked by bath application of 9-phenanthrol (9-phen; 30 μM). ( G ) Summary data showing TICC activity as TRPM4 channel open probability ( NP o ) and ( H ) TICC amplitude in control (n = 12 cells from five mice) and Stim1- smKO (n = 15 cells from five mice) mice (*p<0.05, unpaired t -test). ( I ) Representative conventional whole-cell patch-clamp recordings of 9-phenanthrol–sensitive TRPM4 currents in cerebral artery SMCs from control and Stim1- smKO mice. Currents were activated by free Ca 2+ (200 µM), included in the patch pipette solution, and were recorded using a ramp protocol from –100 to 100 mV from a holding potential of –60 mV. ( J ) Summary of whole-cell TRPM4 current density at +100 mV (control, n = 5 cells from three mice; Stim1- smKO, n = 5 cells from three mice, unpaired t -test). ns, not significant. Figure 7—source data 1. Individual data points and analysis summaries for datasets shown in .

Article Snippet: Antibody , Anti-Stim1 (rabbit polyclonal) , Sigma-Aldrich , Cat# S6072; RRID: AB_1079008 , (1:1000).

Techniques: Patch Clamp, Transferring, Activity Assay

Journal: eLife

Article Title: STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells

doi: 10.7554/eLife.70278

Figure Lengend Snippet: ( A ) Summary data showing vasoconstriction of cerebral pial arteries isolated from control and Stim1- smKO mice in response to 60 mM KCl (n = 12 vessels from six mice for both groups, unpaired t -test). ns, not significant. ( B ) Representative traces showing changes in luminal diameter over a range of intraluminal pressures (5–140 mmHg) in cerebral pial arteries isolated from control (black trace) and Stim1- smKO (red) mice. Gray traces represent passive responses (Ca 2+ -free solution) to changes in intraluminal pressure for each artery. ( C ) Summary data showing myogenic reactivity as a function of intraluminal pressure (n = 6 vessels from three mice for each group; *p<0.05, two-way ANOVA). ( D ) Representative traces showing changes in luminal diameter in response to a range of concentrations (0.1–1,000 nM) of the vasoconstrictor agonist U46619 in cerebral arteries isolated from control (black trace) and Stim1- smKO (red trace) mice. ( E ) Summary data showing vasoconstriction as a function of U46619 concentration (n = 6 vessels from three mice for each group; *p<0.05, two-way ANOVA). ( F ) Summary data showing vasoconstriction of third-order mesenteric arteries isolated from control and Stim1- smKO mice in response to 60 mM KCl (n = 12 vessels from six mice for both groups, unpaired t -test). ns, not significant. ( G ) Representative traces showing changes in luminal diameter over a range of intraluminal pressures (5–140 mmHg) in third-order mesenteric arteries isolated from control (black trace) and Stim1- smKO (red) mice. Gray traces represent passive responses to changes in intraluminal pressure for each artery. ( H ) Summary data for myogenic reactivity as a function of intraluminal pressure (n = 6 vessels from three mice for each group, *p<0.05, two-way ANOVA). ( I ) Representative traces showing changes in luminal diameter in response to a range of concentrations (0.01–100 μM) of the vasoconstrictor agonist phenylephrine (PE) in third-order mesenteric arteries isolated from control (black trace) and Stim1- smKO (red trace) mice. ( J ) Summary data for vasoconstriction as a function of PE concentration, presented as means ± SEM (n = 6 vessels from three mice for each group; *p<0.05, two-way ANOVA). Figure 8—source data 1. Individual data points and analysis summaries for datasets shown in .

Article Snippet: Antibody , Anti-Stim1 (rabbit polyclonal) , Sigma-Aldrich , Cat# S6072; RRID: AB_1079008 , (1:1000).

Techniques: Isolation, Concentration Assay

Journal: eLife

Article Title: STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells

doi: 10.7554/eLife.70278

Figure Lengend Snippet: ( A ) Systolic blood pressure (BP) (mmHg) over 48 hr in conscious, radio telemeter-implanted Myh11 CreERT2 : Stim1 fl/fl mice before (control) and after ( Stim1 -smKO) tamoxifen injection. Shaded regions depict night cycles (n = 5 for both groups; *p<0.05 vs. control day, # p<0.05 vs. control night, paired t -test). ( B ) Diastolic BP measurements for control and Stim1 -smKO mice (n = 5 for both groups, paired t -test). ns, not significant. ( C ) Mean arterial pressure (MAP) for control and Stim1 -smKO mice (n = 5 for both groups, # p<0.05 vs. control night, paired t -test). ns, not significant. ( D ) Pulse pressure for control and Stim1 -smKO mice (n = 5 for both groups; *p<0.05 vs. control day, # p<0.05 vs. control night, paired t -test). ( E ) Heart rate (HR) for control and Stim1 -smKO mice (n = 5 for both groups, paired t -test). ns, not significant. ( F ) Locomotor activity (arbitrary units [AU]) for control and Stim1 -smKO mice (n = 5 for both groups, paired t -test). ns, not significant. 48 hr recordings are shown as means; bar graphs are shown as means ± SEM. Figure 9—source data 1. Individual data points and analysis summaries for datasets shown in .

Article Snippet: Antibody , Anti-Stim1 (rabbit polyclonal) , Sigma-Aldrich , Cat# S6072; RRID: AB_1079008 , (1:1000).

Techniques: Injection, Activity Assay

Journal: eLife

Article Title: STIM1-dependent peripheral coupling governs the contractility of vascular smooth muscle cells

doi: 10.7554/eLife.70278

Figure Lengend Snippet:

Article Snippet: Antibody , Anti-Stim1 (rabbit polyclonal) , Sigma-Aldrich , Cat# S6072; RRID: AB_1079008 , (1:1000).

Techniques: Recombinant, Protease Inhibitor, Bicinchoninic Acid Protein Assay, Software